Select the correct order of steps during PCR.
a. Extension, Denaturation, Annealing
b. Annealing, Extension, Denaturation
c. Denaturation, Annealing, Extension
d. Denaturation, Extension, Annealing
Answer:
c. Denaturation, Annealing, Extension
PCR is based on three steps:
(i) Denaturation: denaturation of the template inot single strands.
(ii) Annealing: annealing of primers to each original strand for new strand synthesis.
(iii) Extension:extension of new DNA strands from the primers.
Select the correct order of steps during PCR.